Purification of DDDDK-tagged protein at a neutral pH to maintain the isolated protein's activities and native conformations.

Background

c-Kit, also known as stem cell factor receptor, steel factor receptor or CD117 is classified as a type III receptor tyrosine kinase (RTK) belonging to the platelet-derived growth factor receptor subfamily. Binding of stem cell factor (SCF), known as c-Kit ligand to c-Kit, initiates autophosphorylation of the receptor, subsequently leading to promotes a signal transduction cascade through Ras-Raf-MAP kinase cascade, phosphatidylinositol- 3-kinase, src family kinases, and JAK/STAT pathways. The roles of c-Kit include maturation of hematopoietic and primordial germ cells precursors and melanocytes during embryonic development. In acute myeloid leukemia (AML), c-Kit has been proposed to play a functional role, and becomes target molecule for drug development.

Purification of DDDDK-tagged protein at a neutral pH to maintain the isolated protein's activities and native conformations.

Background

c-Kit, also known as stem cell factor receptor, steel factor receptor or CD117 is classified as a type III receptor tyrosine kinase (RTK) belonging to the platelet-derived growth factor receptor subfamily. Binding of stem cell factor (SCF), known as c-Kit ligand to c-Kit, initiates autophosphorylation of the receptor, subsequently leading to promotes a signal transduction cascade through Ras-Raf-MAP kinase cascade, phosphatidylinositol- 3-kinase, src family kinases, and JAK/STAT pathways. The roles of c-Kit include maturation of hematopoietic and primordial germ cells precursors and melanocytes during embryonic development. In acute myeloid leukemia (AML), c-Kit has been proposed to play a functional role, and becomes target molecule for drug development.