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MBL Life Sciences
MBL Life Sciences
Monoclonal antibody of 100 µl targeting IL-18 for IP.
Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
This antibody was purified from hybridoma (clone 159-12B) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with SD rat splenocyte immunized with recombinant human IL-18.
| Product Specifications | |
| Application | IP |
| Reactivity | Human |
| Clonality | Monoclonal IgG2a |
| Host | Rat |
| Immunogen | Recombinant human IL-18 |
| Gene ID - Human | |
| Formulation | 100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Storage | -20C |
| Documents & Links for Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) | |
| Datasheet | Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) Datasheet |
| Documents & Links for Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) | |
| Datasheet | Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) Datasheet |
| Citations for Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) – 0 Found |
| Dao, T., et al.,. Cell Immunology. 1996;(173):230-235. |
| Micallef, M., et al. Eur. J. Immunol. 1996;26:1647-1651. |
| Ushio, S., et al.. J. Immunol. . 1996;156:4274-4279. |
| Okamura, H., et al.,. Nature. 1995;378:88-91. |
Monoclonal antibody of 100 µl targeting IL-18 for IP.
Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
This antibody was purified from hybridoma (clone 159-12B) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with SD rat splenocyte immunized with recombinant human IL-18.
| Product Specifications | |
| Application | IP |
| Reactivity | Human |
| Clonality | Monoclonal IgG2a |
| Host | Rat |
| Immunogen | Recombinant human IL-18 |
| Gene ID - Human | |
| Formulation | 100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Storage | -20C |
| Documents & Links for Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) | |
| Datasheet | Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) Datasheet |
| Documents & Links for Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) | |
| Datasheet | Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) Datasheet |
| Citations for Anti-IL-18 (Clone 159-12B) (Human) mAb (Clone: 159-12B) – 0 Found |
| Dao, T., et al.,. Cell Immunology. 1996;(173):230-235. |
| Micallef, M., et al. Eur. J. Immunol. 1996;26:1647-1651. |
| Ushio, S., et al.. J. Immunol. . 1996;156:4274-4279. |
| Okamura, H., et al.,. Nature. 1995;378:88-91. |
