Background

Advanced Glycation End Product (AGE) is a general term for structures generated in the late stage of non-enzymatic glycation reactions (Millard reactions) between reducing sugars and proteins. AGE accumulation is known to be significantly elevated in age-related diseases such as diabetes and arteriosclerosis.

Nω-(carboxymethyl) arginine (CMA), a CML analogue, is an acid-labile AGE structure which was discovered in enzymatic hydrolysate of glycated collagen. CMA is preferentially generated in glycated collagen.

Source: Professor Nagai Ryuji, Tokai University Faculty of Agriculture Department of Biosciences Food Bioregulation Research Laboratory

Application

Western blotting, 1/100 to 1/200
Immunohistochemistry (frozen sections), 1/50 to 1/100
ELISA, 1/100 to 1/200

References

1) Iijima K, Murata M, Takahara H, Irie S, Fujimoto D. Identification of N(omega)-carboxymethylarginine as a novel acid-labileadvanced glycation end product in collagen. Biochem J. 347 Pt 1:23-27 (2000) PMID: 10727397

2) Mera K., Fujiwara Y., Otagiri M., Sakata N., Nagai R. Immunological Detection of Nε -carboxymethylarginine by Specific Antibody. Ann N Y Acad Sci. 1126, 155-157 (2008) PMID: 18079475

Background

Advanced Glycation End Product (AGE) is a general term for structures generated in the late stage of non-enzymatic glycation reactions (Millard reactions) between reducing sugars and proteins. AGE accumulation is known to be significantly elevated in age-related diseases such as diabetes and arteriosclerosis.

Nω-(carboxymethyl) arginine (CMA), a CML analogue, is an acid-labile AGE structure which was discovered in enzymatic hydrolysate of glycated collagen. CMA is preferentially generated in glycated collagen.

Source: Professor Nagai Ryuji, Tokai University Faculty of Agriculture Department of Biosciences Food Bioregulation Research Laboratory

Application

Western blotting, 1/100 to 1/200
Immunohistochemistry (frozen sections), 1/50 to 1/100
ELISA, 1/100 to 1/200

References

1) Iijima K, Murata M, Takahara H, Irie S, Fujimoto D. Identification of N(omega)-carboxymethylarginine as a novel acid-labileadvanced glycation end product in collagen. Biochem J. 347 Pt 1:23-27 (2000) PMID: 10727397

2) Mera K., Fujiwara Y., Otagiri M., Sakata N., Nagai R. Immunological Detection of Nε -carboxymethylarginine by Specific Antibody. Ann N Y Acad Sci. 1126, 155-157 (2008) PMID: 18079475