Product Description

Mesenchymal stem cells (MSCs), a type of somatic stem cell, can be isolated from adipose tissue, bone marrow, umbilical cord, and dental pulp etc. MSCs are known for their excellent self-proliferation abilities, and possess the capacity to differentiate into a variety of cells, including not only bone, cartilage, and fat, but also liver2, 3, 4, 5). MSCs do not express MHC Class-II proteins3); possess immunomodulatory functions 6) ; and accumulate at diseased sites, secreting various cytokines and growth factors4), which promotes tissue regeneration and repair 5, 7). Traditionally, bone marrow-derived MSCs (BMMSCs) have been used for research; however, isolation of MSCs from mouse bone marrow has been extremely challenging due to poor purity and yield, and due to contamination with hematopoietic cells, which can inhibit MSC proliferation8) . In contrast, compact bone-derived MSCs (CBMSCs) are isolated by enzymatic processing of bone fragments from mouse compact bone, followed by culturing and isolating cells with minimal hematopoietic contamination, thus maintaining their multipotency and high proliferative capacity8) for various research purposes. This product consists of cells that have been subcultured (two passages) after being isolated from the compact bone of adult SD rat treated with collagenase. This product has been confirmed to differentiate into osteocytes, adipocytes, and chondrocytes (Note: Verification of surface antigen markers has not been conducted.)

Product Description

Mesenchymal stem cells (MSCs), a type of somatic stem cell, can be isolated from adipose tissue, bone marrow, umbilical cord, and dental pulp etc. MSCs are known for their excellent self-proliferation abilities, and possess the capacity to differentiate into a variety of cells, including not only bone, cartilage, and fat, but also liver2, 3, 4, 5). MSCs do not express MHC Class-II proteins3); possess immunomodulatory functions 6) ; and accumulate at diseased sites, secreting various cytokines and growth factors4), which promotes tissue regeneration and repair 5, 7). Traditionally, bone marrow-derived MSCs (BMMSCs) have been used for research; however, isolation of MSCs from mouse bone marrow has been extremely challenging due to poor purity and yield, and due to contamination with hematopoietic cells, which can inhibit MSC proliferation8) . In contrast, compact bone-derived MSCs (CBMSCs) are isolated by enzymatic processing of bone fragments from mouse compact bone, followed by culturing and isolating cells with minimal hematopoietic contamination, thus maintaining their multipotency and high proliferative capacity8) for various research purposes. This product consists of cells that have been subcultured (two passages) after being isolated from the compact bone of adult SD rat treated with collagenase. This product has been confirmed to differentiate into osteocytes, adipocytes, and chondrocytes (Note: Verification of surface antigen markers has not been conducted.)