Academic users: Please submit a completed MTA form with your order to orders@cosmobiousa.com.
Commercial users: Please contact us at orders@cosmobiousa.com before placing an order. A license agreement with MBL International will need to be completed for ordering.

Product Description

This plasmid is designed for expression of Mitochondria-targeted CoralHue^™ monomeric Keima-Red (MT-targeted mKeima-Red) in mammalian cells. CoralHueTM Keima-Red has been cloned from the stony coral, whose Japanese name is “Komon-Sango”. A monomeric version of CoralHueTM Keima-Red (mKeima-Red) absorbs light maximally at 440 nm and emits red light at 620 nm with a large Storks-shift, providing another option for multicolor fluorescence analyses. Targeting of mKeima-Red to the mitochondria is achieved with the signal peptide fused to the N-terminus of mKeima-Red.

Citations 

1. Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016) (PMID:27903607)

1. Hirota Y et al. Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways. Autophagy. 11, 332–343 (2015)(PMID:25831013)

1. Zhou H et al. Pathogenesis of cardiac ischemia reperfusion injury is associated with CK2α-disturbed mitochondrial homeostasis via suppression of FUNDC1-related mitophagy. Cell Death Differ. 25, 1080-1093 (2018)(PMID:29540794)

1. Safiulina D et al. Miro proteins prime mitochondria for Parkin translocation and mitophagy. EMBO J. 38, pii: e99384 (2019)(PMID:30504269)

1. Zhu H et al. Phosphoglycerate mutase 5 exacerbates cardiac ischemia-reperfusion injury through disrupting mitochondrial quality control. Redox Biol. 38, 101777 (2021)(PMID:33166869)

Flow Cytometry

1. Alcalá S et al. ISG15 and ISGylation is required for pancreatic cancer stem cell mitophagy and metabolic plasticity. Nat Commun. 11, 2682 (2020)(PMID:32472071)

Immunocytochemistry

1. Wang J et al. Fundc1-dependent Mitophagy Is Obligatory to Ischemic Preconditioning-Conferred Renoprotection in Ischemic AKI via Suppression of Drp1-mediated Mitochondrial Fission. Redox Biol. 30, :101415 (2020) (PMID:31901590)
2. Wang J et al. Bax Inhibitor 1 Preserves Mitochondrial Homeostasis in Acute Kidney Injury Through Promoting Mitochondrial Retention of PHB2. Theranostics. 10, 384-397 (2020)(PMID:31903127)

References

1. Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat Biotechnol. 24, 577-81 (2006)（PMID: 16648840）

Academic users: Please submit a completed MTA form with your order to orders@cosmobiousa.com.
Commercial users: Please contact us at orders@cosmobiousa.com before placing an order. A license agreement with MBL International will need to be completed for ordering.

Product Description

This plasmid is designed for expression of Mitochondria-targeted CoralHue^™ monomeric Keima-Red (MT-targeted mKeima-Red) in mammalian cells. CoralHueTM Keima-Red has been cloned from the stony coral, whose Japanese name is “Komon-Sango”. A monomeric version of CoralHueTM Keima-Red (mKeima-Red) absorbs light maximally at 440 nm and emits red light at 620 nm with a large Storks-shift, providing another option for multicolor fluorescence analyses. Targeting of mKeima-Red to the mitochondria is achieved with the signal peptide fused to the N-terminus of mKeima-Red.

Citations 

1. Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016) (PMID:27903607)

1. Hirota Y et al. Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways. Autophagy. 11, 332–343 (2015)(PMID:25831013)

1. Zhou H et al. Pathogenesis of cardiac ischemia reperfusion injury is associated with CK2α-disturbed mitochondrial homeostasis via suppression of FUNDC1-related mitophagy. Cell Death Differ. 25, 1080-1093 (2018)(PMID:29540794)

1. Safiulina D et al. Miro proteins prime mitochondria for Parkin translocation and mitophagy. EMBO J. 38, pii: e99384 (2019)(PMID:30504269)

1. Zhu H et al. Phosphoglycerate mutase 5 exacerbates cardiac ischemia-reperfusion injury through disrupting mitochondrial quality control. Redox Biol. 38, 101777 (2021)(PMID:33166869)

Flow Cytometry

1. Alcalá S et al. ISG15 and ISGylation is required for pancreatic cancer stem cell mitophagy and metabolic plasticity. Nat Commun. 11, 2682 (2020)(PMID:32472071)

Immunocytochemistry

1. Wang J et al. Fundc1-dependent Mitophagy Is Obligatory to Ischemic Preconditioning-Conferred Renoprotection in Ischemic AKI via Suppression of Drp1-mediated Mitochondrial Fission. Redox Biol. 30, :101415 (2020) (PMID:31901590)
2. Wang J et al. Bax Inhibitor 1 Preserves Mitochondrial Homeostasis in Acute Kidney Injury Through Promoting Mitochondrial Retention of PHB2. Theranostics. 10, 384-397 (2020)(PMID:31903127)

References

1. Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat Biotechnol. 24, 577-81 (2006)（PMID: 16648840）