Loading...
Loading...
Loading...
Loading...
Cosmo Bio
Measurement Kit for D-Serine in urine and food samples
D-form amino acids have long been known as components of bacterial cell wall peptidoglycan layers. More recent developments in analytical technologies have demonstrated that D-amino acids are also present in mammals and display specificand important physiological activities. Particularly high levels of D-serine levels are present in brain tissue where D-serine functions as an important co-agonist of N-methyl-D-aspartate (NMDA) receptors (NMDR), involved in regulating higher brainfunction such as memory and learning. D-serine is suggested to play a role in neurodegeneration associated with diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS). High levels of free D-serine can also be found in humanurine although its significance is unclear.
D-serine is commonly assayed by HPLC or GC following conversion of D-serine to diastereomer derivatives. Such methods are time consuming, require expensive instrumentation, and are not suitable for processing large numbers of samples. The D-Serine Colorimetric Assay Kit employs the D-form specific enzyme D-serine dehydratase from Saccharomyces cerevisiae (DsdSC) enabling the quantitation of D-serine by spectrophotometric measurement.
DsdSC catalyzes the conversion of D-serine to pyruvate and ammonia. In the presence of lactate dehydrogenase (LDH), pyruvate is reduced to lactate with the concomitant oxidation of NADH to NAD. The reaction can be monitored by measuring the decrease in absorbance at 340nm due to the oxidation of NADH. The D-serine concentration in unknown samples is determined by comparison with a standard curve.
Urine
| Documents & Links for D-Serine Colorimetric Assay Kit | |
| Datasheet | D-Serine Colorimetric Assay Kit Datasheet |
| Flyer | D-Serine Colorimetric Assay Kit Flyer |
| Documents & Links for D-Serine Colorimetric Assay Kit | |
| Datasheet | D-Serine Colorimetric Assay Kit Datasheet |
| Flyer | D-Serine Colorimetric Assay Kit Flyer |
| Citations for D-Serine Colorimetric Assay Kit – 2 Found |
| Hirohashi, Noritaka; Tamura-Nakano, Miwa; Nakaya, Fumio; Iida, Tomohiro; Iwata, Yoko. Sneaker Male Squid Produce Long-lived Spermatozoa by Modulating Their Energy Metabolism. The Journal Of Biological Chemistry. 2016;291(37):19324-34. PubMed |
| Qi, Liang; Cui, En-Hui; Ji, Chun-Mei; Zhang, Xiao-Bing; Wang, Ze-Ai; Sun, Yuan-Zhao; Xu, Jian-Chang; Zhai, Xiao-Fu; Chen, Zhong-Jun; Li, Jing; Zheng, Jin-Yu; Yu, Ru-Tong. Specific knockdown of hippocampal astroglial EphB2 improves synaptic function via inhibition of D-serine secretion in APP/PS1 mice. American Journal Of Translational Research. 11(2):1073-1083. PubMed |
Try browsing our complete catalog of products.
Cosmo Bio
Measurement Kit for D-Serine in urine and food samples
D-form amino acids have long been known as components of bacterial cell wall peptidoglycan layers. More recent developments in analytical technologies have demonstrated that D-amino acids are also present in mammals and display specificand important physiological activities. Particularly high levels of D-serine levels are present in brain tissue where D-serine functions as an important co-agonist of N-methyl-D-aspartate (NMDA) receptors (NMDR), involved in regulating higher brainfunction such as memory and learning. D-serine is suggested to play a role in neurodegeneration associated with diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS). High levels of free D-serine can also be found in humanurine although its significance is unclear.
D-serine is commonly assayed by HPLC or GC following conversion of D-serine to diastereomer derivatives. Such methods are time consuming, require expensive instrumentation, and are not suitable for processing large numbers of samples. The D-Serine Colorimetric Assay Kit employs the D-form specific enzyme D-serine dehydratase from Saccharomyces cerevisiae (DsdSC) enabling the quantitation of D-serine by spectrophotometric measurement.
DsdSC catalyzes the conversion of D-serine to pyruvate and ammonia. In the presence of lactate dehydrogenase (LDH), pyruvate is reduced to lactate with the concomitant oxidation of NADH to NAD. The reaction can be monitored by measuring the decrease in absorbance at 340nm due to the oxidation of NADH. The D-serine concentration in unknown samples is determined by comparison with a standard curve.
Urine
| Documents & Links for D-Serine Colorimetric Assay Kit | |
| Datasheet | D-Serine Colorimetric Assay Kit Datasheet |
| Flyer | D-Serine Colorimetric Assay Kit Flyer |
| Documents & Links for D-Serine Colorimetric Assay Kit | |
| Datasheet | D-Serine Colorimetric Assay Kit Datasheet |
| Flyer | D-Serine Colorimetric Assay Kit Flyer |
| Citations for D-Serine Colorimetric Assay Kit – 2 Found |
| Hirohashi, Noritaka; Tamura-Nakano, Miwa; Nakaya, Fumio; Iida, Tomohiro; Iwata, Yoko. Sneaker Male Squid Produce Long-lived Spermatozoa by Modulating Their Energy Metabolism. The Journal Of Biological Chemistry. 2016;291(37):19324-34. PubMed |
| Qi, Liang; Cui, En-Hui; Ji, Chun-Mei; Zhang, Xiao-Bing; Wang, Ze-Ai; Sun, Yuan-Zhao; Xu, Jian-Chang; Zhai, Xiao-Fu; Chen, Zhong-Jun; Li, Jing; Zheng, Jin-Yu; Yu, Ru-Tong. Specific knockdown of hippocampal astroglial EphB2 improves synaptic function via inhibition of D-serine secretion in APP/PS1 mice. American Journal Of Translational Research. 11(2):1073-1083. PubMed |
No reviews have been added for this product.