Description: KOMA Biotech launched its new EzWay™ Mouse Tail Direct PCR Kit, which is designed to enable DNA amplification directly from mouse tail without DNA extraction. Just cut the mouse tail and incubate it in the lysis buffer provided in the kit for 10 minutes and then use 1ul of lysate for PCR. It doesn't require overnight incubation or Proteinase K (PK) treatment as well as DNA purification process. Direct Taq PCR MasterMix in this kit is a convenient mixture of Taq enzyme, dNTP, MgCl2, dye, additives and Direct PCR Buffer, so you can just add the tail lysate and primers, and then go for PCR. This kit will save your enormous amounts of material, cost and time from conventional genotyping methods. Also, it reduces risk of handling errors and contamination
Features: Genotyping of transgenic mouse using tail or ear tissue
Fast and simple method for genotyping without DNA purification
Just 10 minutes incubation required
No need to add Proteinase K
Saving enormous amounts of material and time
Optimized MasterMix type containing Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye and additives
Direct loading of PCR products without adding red dye
No. of Applications : 100 reactions at 20ul PCR volume
Applications: Mouse Tail PCR
Description: KOMA Biotech launched its new EzWay™ Mouse Tail Direct PCR Kit, which is designed to enable DNA amplification directly from mouse tail without DNA extraction. Just cut the mouse tail and incubate it in the lysis buffer provided in the kit for 10 minutes and then use 1ul of lysate for PCR. It doesn't require overnight incubation or Proteinase K (PK) treatment as well as DNA purification process. Direct Taq PCR MasterMix in this kit is a convenient mixture of Taq enzyme, dNTP, MgCl2, dye, additives and Direct PCR Buffer, so you can just add the tail lysate and primers, and then go for PCR. This kit will save your enormous amounts of material, cost and time from conventional genotyping methods. Also, it reduces risk of handling errors and contamination
Features: Genotyping of transgenic mouse using tail or ear tissue
Fast and simple method for genotyping without DNA purification
Just 10 minutes incubation required
No need to add Proteinase K
Saving enormous amounts of material and time
Optimized MasterMix type containing Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye and additives
Direct loading of PCR products without adding red dye
No. of Applications : 100 reactions at 20ul PCR volume
