Product Description
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones.
For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 149
| Product Specifications |
| Application |
FC |
| Reactivity |
Mouse |
| Conjugation |
PE |
| Immunogen |
β-galactosidase |
| Peptide Sequence |
DAPIYTNV |
| Class |
Class I |
| Sequence Location |
96-103 |
| MHC Allele |
H-2Kb |
| Citations for H-2Kb β-galactosidase Tetramer-DAPIYTNV-PE – 1 Found |
| Hemmi, Masahisa; Tachibana, Masashi; Fujimoto, Natsuki; Shoji, Masaki; Sakurai, Fuminori; Kobiyama, Kouji; Ishii, Ken J; Akira, Shizuo; Mizuguchi, Hiroyuki. T Helper 17 Promotes Induction of Antigen-Specific Gut-Mucosal Cytotoxic T Lymphocytes following Adenovirus Vector Vaccination. Frontiers In Immunology. 8:1456. PubMed |
