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MBL Life Sciences
MBL Life Sciences
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 206
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | APC |
| Immunogen | islet-specific glucose-6-phosphatase catalytic subunit related protein |
| Peptide Sequence | VYLKTNVFL |
| Class | Class I |
| Sequence Location | 206-214 |
| MHC Allele | H-2Kd |
| Documents & Links for H-2Kd IGRP Tetramer-VYLKTNVFL-APC | |
| Datasheet | H-2Kd IGRP Tetramer-VYLKTNVFL-APC Datasheet |
| Documents & Links for H-2Kd IGRP Tetramer-VYLKTNVFL-APC | |
| Datasheet | H-2Kd IGRP Tetramer-VYLKTNVFL-APC Datasheet |
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 206
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | APC |
| Immunogen | islet-specific glucose-6-phosphatase catalytic subunit related protein |
| Peptide Sequence | VYLKTNVFL |
| Class | Class I |
| Sequence Location | 206-214 |
| MHC Allele | H-2Kd |
| Documents & Links for H-2Kd IGRP Tetramer-VYLKTNVFL-APC | |
| Datasheet | H-2Kd IGRP Tetramer-VYLKTNVFL-APC Datasheet |
| Documents & Links for H-2Kd IGRP Tetramer-VYLKTNVFL-APC | |
| Datasheet | H-2Kd IGRP Tetramer-VYLKTNVFL-APC Datasheet |
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