Product Description

The HA epitope tag, YPYDVPDYA, is derived from Human influenza hemaglutinin (HA) 98-106 aa. This HA tag is also commonly used in such as mammalian cell expression vectors. MBL's HA-tagged Protein PURIFICATION KIT is designed for the isolation of HA-tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBL has developed the Anti-HA tag Beads to purify HA-tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of HA-tagged proteins from the Beads is achieved by the addition of the HA peptide. As the HA peptide competes with HA-tagged proteins on the Beads, the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency has optimized the simple procedure of this kit.

Quick and efficient purification of HA-tagged protein at a neutral pH to maintain the isolated protein's activities and native conformations.

References

1. Palzer S et al. An expanded genetic code in Candida albicans to study protein-protein interactions in vivo. Eukaryot Cell. 12, 816-827 (2013)(PMID:23543672)

1. Takahashi RU et al. Loss of microRNA-27b contributes to breast cancer stem cell generation by activating ENPP1. Nat Commun. 6, 7318 (2015)(PMID:26065921)

1. Murai N et al. Novel ubiquitin-independent nucleolar c-Myc degradation pathway mediated by antizyme 2. Sci Rep. 8, 3005 (2018)(PMID:29445227)

1. Nellaepalli S et al. The photosystem I assembly apparatus consisting of Ycf3-Y3IP1 and Ycf4 modules. Nat Commun. 9, 2439 (2018)(PMID:29934511)

Product Description

The HA epitope tag, YPYDVPDYA, is derived from Human influenza hemaglutinin (HA) 98-106 aa. This HA tag is also commonly used in such as mammalian cell expression vectors. MBL's HA-tagged Protein PURIFICATION KIT is designed for the isolation of HA-tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBL has developed the Anti-HA tag Beads to purify HA-tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of HA-tagged proteins from the Beads is achieved by the addition of the HA peptide. As the HA peptide competes with HA-tagged proteins on the Beads, the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency has optimized the simple procedure of this kit.

Quick and efficient purification of HA-tagged protein at a neutral pH to maintain the isolated protein's activities and native conformations.

References

1. Palzer S et al. An expanded genetic code in Candida albicans to study protein-protein interactions in vivo. Eukaryot Cell. 12, 816-827 (2013)(PMID:23543672)

1. Takahashi RU et al. Loss of microRNA-27b contributes to breast cancer stem cell generation by activating ENPP1. Nat Commun. 6, 7318 (2015)(PMID:26065921)

1. Murai N et al. Novel ubiquitin-independent nucleolar c-Myc degradation pathway mediated by antizyme 2. Sci Rep. 8, 3005 (2018)(PMID:29445227)

1. Nellaepalli S et al. The photosystem I assembly apparatus consisting of Ycf3-Y3IP1 and Ycf4 modules. Nat Commun. 9, 2439 (2018)(PMID:29934511)