Purpose of Use:
Hematoxylin-Eosin is a common staining technique used in a wide range of tissues. Through this method, nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple. Harris Hematoxylin does not contain mercury.

Use and sample preparation:
Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

Components:

• Harris Hematoxylin Reagent
• Eosin Reagent

Short procedure:

1. Add Harris Hematoxylin
2. Wash with tap water
3. Add Glacial acetic acid
4. Wash with tap water
5. Add Eosin solution
6. Wash with tap water
7. Dehydrate with graded ethanol, clear and coverslip

Staining characteristics:
Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple.