Product Description

T lymphocytes play a central role in immune system. Total T cell and T cell subset counts are measured by detection of various cell surface molecules. Enumeration of CD8+ antigen-specific T cells requires cognate recognition of the T cell receptor (TCR) by a class I MHC/peptide complex.　This can be done using T-Select MHC class I Tetramers which are composed of four MHC class I molecules each bound to the specific peptide and conjugated with a fluorescent protein. Thus, T-Select MHC Tetramer assays allow quantitation of the total T cell population specific for a given peptide complexed with a particular MHC molecule. Furthermore, since binding does not depend on functional pathways, this population includes specific CD8+ T cells regardless of functional status. Measurements may be performed in whole blood or isolated lymphocyte/mononuclear cell preparations. In some cases where frequency is low, it may be necessary to perform an in vitro cell expansion. Specific cell staining is accomplished by incubating the sample with the T-Select MHC Tetramer reagent, then washing away excess Tetramer. The number of Tetramer positive lymphocytes is then determined by flow cytometry. These Tetramer reagents comprise human class I HLA-A*02:01 and a nonamer peptide based on predictions of peptide binding to MHC class I molecules (e.g. IEDB Analysis Resource), and are useful as a negative control of Tetramer reagents.

Product Description

T lymphocytes play a central role in immune system. Total T cell and T cell subset counts are measured by detection of various cell surface molecules. Enumeration of CD8+ antigen-specific T cells requires cognate recognition of the T cell receptor (TCR) by a class I MHC/peptide complex.　This can be done using T-Select MHC class I Tetramers which are composed of four MHC class I molecules each bound to the specific peptide and conjugated with a fluorescent protein. Thus, T-Select MHC Tetramer assays allow quantitation of the total T cell population specific for a given peptide complexed with a particular MHC molecule. Furthermore, since binding does not depend on functional pathways, this population includes specific CD8+ T cells regardless of functional status. Measurements may be performed in whole blood or isolated lymphocyte/mononuclear cell preparations. In some cases where frequency is low, it may be necessary to perform an in vitro cell expansion. Specific cell staining is accomplished by incubating the sample with the T-Select MHC Tetramer reagent, then washing away excess Tetramer. The number of Tetramer positive lymphocytes is then determined by flow cytometry. These Tetramer reagents comprise human class I HLA-A*02:01 and a nonamer peptide based on predictions of peptide binding to MHC class I molecules (e.g. IEDB Analysis Resource), and are useful as a negative control of Tetramer reagents.