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Kohjin Bio
Kohjin Bio
Serum-Free Media for Mesenchymal Stem Cells
Cell culture example Using KBM ADSC-2+2% serum substitute, cell proliferation tests of adipose-derived stem cells cultured to P2, confirmation of cell surface markers after five passages and differentiation induction tests were carried out. It was also confirmed that the proliferation performance was almost equivalent in umbilical cord-derived stem cells.
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| Fig.1:Growth curve Fig.2:Variation of PDT | Fig.3:Cell morphology at P5 | Table.1: Ratio of cell surface markers at P5 |
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| Fig.4: Differentiation potential confirmation test of MSC at P6 a) Adipose cells (Oil red O stain) b) Osteoblastic cells (Alizarin Red S stain) c) Chondrocytes (Alcian Blue stain) |
Fig 5: Culture test data of Umbilical cord-derived MSC a) Cell morphology at P5 b) PDL at passage |
| Documents & Links for KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) | |
| Datasheet | KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) Datasheet |
| Documents & Links for KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) | |
| Datasheet | KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) Datasheet |
Serum-Free Media for Mesenchymal Stem Cells
Cell culture example Using KBM ADSC-2+2% serum substitute, cell proliferation tests of adipose-derived stem cells cultured to P2, confirmation of cell surface markers after five passages and differentiation induction tests were carried out. It was also confirmed that the proliferation performance was almost equivalent in umbilical cord-derived stem cells.
|
|
|
| Fig.1:Growth curve Fig.2:Variation of PDT | Fig.3:Cell morphology at P5 | Table.1: Ratio of cell surface markers at P5 |
|
|
| Fig.4: Differentiation potential confirmation test of MSC at P6 a) Adipose cells (Oil red O stain) b) Osteoblastic cells (Alizarin Red S stain) c) Chondrocytes (Alcian Blue stain) |
Fig 5: Culture test data of Umbilical cord-derived MSC a) Cell morphology at P5 b) PDL at passage |
| Documents & Links for KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) | |
| Datasheet | KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) Datasheet |
| Documents & Links for KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) | |
| Datasheet | KBM ADSC-5 (Serum-Free Media for Mesenchymal Stem Cells) Datasheet |