Fatty acid-free bovine serum albumin (BSA) (0.05 g/ml) was incubated with 50 mM of glucose in a 0.05 M sodium phosphate buffer (pH 7.4) at 37°C for 24 weeks, followed by dialysis against PBS. The CML content (0.4 mol CML/mol BSA) was determined by amino acid analysis. This method is prepared mildly-modified-AGE-BSA (mild-AGE-BSA). The CML content for the mild-AGE-BSA is  24.4 mmol CML/mol Lys. However, the CML contents for the diabetic (DM)- and non-diabetic  human lens samples were about 17.4 mmol /mol Lys and about 8.6 mmol/mol Lys, respectively,  thus indicating that the CML content of mild-AGE-BSA was similar to physiological samples.

Volume: 200 ul

Concentration: 1 mg/ml

Storage: Store below -20°C (below -70°C for prolonged storage).  Aliquot to avoid cycles of freeze/thaw.

Fatty acid-free bovine serum albumin (BSA) (0.05 g/ml) was incubated with 50 mM of glucose in a 0.05 M sodium phosphate buffer (pH 7.4) at 37°C for 24 weeks, followed by dialysis against PBS. The CML content (0.4 mol CML/mol BSA) was determined by amino acid analysis. This method is prepared mildly-modified-AGE-BSA (mild-AGE-BSA). The CML content for the mild-AGE-BSA is  24.4 mmol CML/mol Lys. However, the CML contents for the diabetic (DM)- and non-diabetic  human lens samples were about 17.4 mmol /mol Lys and about 8.6 mmol/mol Lys, respectively,  thus indicating that the CML content of mild-AGE-BSA was similar to physiological samples.

Volume: 200 ul

Concentration: 1 mg/ml

Storage: Store below -20°C (below -70°C for prolonged storage).  Aliquot to avoid cycles of freeze/thaw.