Nicotinamide adenine dinucleotide (NAD) is a well-known coenzyme found in all living cells that plays an important role in energy metabolism. NAD exists as an oxidized form (NAD+) and a reduced form (NADH). The NAD+/NADH redox couple is essential for maintaining cellular redox homeostasis and for modulating numerous biological events, including cellular metabolism. Prolonged disequilibrium of NAD+/NADH balance disturbs the physiological functions, resulting in several diseases like metabolic diseases, neurodegeneration disorders or cancer.
In addition to its vital role as a coenzyme in energy metabolism, NAD+ serves as substrate for three classes of enzymes: sirtuin family deacetylases, poly(ADP)-ribosyl polymerases and cADP-ribose synthases.

BQC NAD+/NADH Determination Assay Kit is a quick, easy, and reproducible assay for quantifying NAD+/NADH concentration in several biological samples.