The kit is designed for nitrite/nitrate determination in biological samples.

Background

The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process. Regarding nitrosative stress, high levels of iNOS have been found in various inflammatory diseases such as arthritis and obesity, and increased levels of NO have been also associated to other cardiovascular diseases. Bioquochem Nitrate/Nitrite Determination Kit is recommended for the determination of nitrite and nitrate, which is a method for the detection of nitric oxide formation. The assay described here measures the nitrite and nitrate anions. Firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences*. The detection is based on the final product detection (diazonium compound, λmax= 540 nm) obtained after nitrite reaction in several steps with sulfanilamide.

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