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Best US Prices...BY FAR! OptiPrep™ is a sterile, endotoxin-tested, multi-purpose separation medium of 60% iodixanol in water with a density of 1.32 g/ml, ideal for isolating viruses, subcellular organelles, membranes, macromolecules and cells, especially when controlling osmolality is a priority. Iodixanol has undergone rigorous clinical testing and is manufactured under strict EU cGMP compliance.

Product Description

Specifications

Iodixanol 60% (w/v)
Density 1.320 ± 0.001 g/ml
Osmolality ~170 mOsm
Endotoxin < 1.0 EU/ml

Applications

Membrane trafficking and cell signaling analysis
Endocytosis and exocytosis analysis
Mammalian and non-mammalian cell purification; routinely achieve maximum yield of non-human lymphocytes with no neutrophil contamination
Mammalian and nonmammalian organelle purification
Plasma membrane and domain purification
Membrane vesicle and cytosol purification
Virus purification
Plasma lipoprotein purification
Protein and protein complex purification
Plasmid DNA purification
Ribonucleoprotein purification

Features and properties

Developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing.
Non-ionic
Non-toxic to cells
Metabolically inert.
Biologically and physiochemically very similar to Nycodenz® with some key differences:
- Structurally, an approximate dimer of Nycodenz®
- Osmolality approximately half that of a Nycodenz® solution of the same density
- Can be made iso-osmotic at all densities
- Can be added directly to a cell or organelle suspension without changing its osmolality, facilitating purification in flotation gradients
- Forms self-generated gradients more quickly than Nycodenz® due to higher molecular mass
High viability of gradient-purified mammalian and non-mammalian cells
Effective organelle and membrane vesicle fractionation using either pre-formed or self-generated gradients
Virus infectivity at least 100x higher than from CsCl gradients
Better preservation of protein-protein and protein-nucleic acid complexes than with sucrose or glycerol gradients
Plasma lipoproteins analyzed in self-generated gradients within 4h
Sterility is claimed according to Ph. Eur.
Manufactured, packaged and released in compliance with:
- Current EU Guide to Good Manufacturing Practice
- Fresenius Kabi AS Quality System
- Fresenius Kabi AS Manufacturing License

Documents & Links for OptiPrep - Density Gradient Media (Iodixanol)
Datasheet	OptiPrep - Density Gradient Media (Iodixanol) Datasheet
MSDS	OptiPrep MSDS
Flyer	OptiPrep Leaflet 2021
Flyer	Optiprep Leaflet - Analysis of membane trafficking
Flyer	Optiprep Leaflet - Isolation of blood cells
Flyer	Optiprep Leaflet - Isolation of Cells
Flyer	Optiprep Leaflet - Isolation of Exosomes
Flyer	Optiprep Leaflet - Isolation of macromolecules and lipoproteins
Flyer	Optiprep Leaflet - Isolation of Plasma Membranes
Flyer	Optiprep Leaflet - Isolation of Cell Organelles
Flyer	Optiprep Leaflet - Isolation of Viruses
Flyer	OptiPrep - Density Gradient Media (Iodixanol) Flyer

Documents & Links for OptiPrep - Density Gradient Media (Iodixanol)
Datasheet	OptiPrep - Density Gradient Media (Iodixanol) Datasheet
MSDS	OptiPrep MSDS
Flyer	OptiPrep Leaflet 2021
Flyer	Optiprep Leaflet - Analysis of membane trafficking
Flyer	Optiprep Leaflet - Isolation of blood cells
Flyer	Optiprep Leaflet - Isolation of Cells
Flyer	Optiprep Leaflet - Isolation of Exosomes
Flyer	Optiprep Leaflet - Isolation of macromolecules and lipoproteins
Flyer	Optiprep Leaflet - Isolation of Plasma Membranes
Flyer	Optiprep Leaflet - Isolation of Cell Organelles
Flyer	Optiprep Leaflet - Isolation of Viruses
Flyer	OptiPrep - Density Gradient Media (Iodixanol) Flyer

Citations for OptiPrep - Density Gradient Media (Iodixanol) – 1 Found

Yang, Yang; Wang, Jing; Shigematsu, Hideki; Xu, Weiming; Shih, William M; Rothman, James E; Lin, Chenxiang. Self-assembly of size-controlled liposomes on DNA nanotemplates. Nature Chemistry. 2016;8(5):476-83. PubMed

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OptiPrep - Density Gradient Media (Iodixanol)

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OptiPrep - Density Gradient Media (Iodixanol)

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OptiPrep - Density Gradient Media (Iodixanol)

Product Description

Additional information

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OptiPrep - Density Gradient Media (Iodixanol)

Product Description

Additional information

Specifications

Product Description

Specifications

Product Description