Product Description

This product contains two DNA cassettes for detecting CDK4-Cyclin D1 interaction in living cells. Fluoppi is a technology providing an easy way to visualize protein-protein interactions (PPIs) with a high signal to noise ratio. It employs an oligomeric assembly helper tag (Ash-tag) and a tetrameric fluorescent protein tag (FP-tag) to create detectable fluorescent puncta when there are interactions between two proteins fused to the tags. Genetic fusion of protein X with FP-tag, and Y with Ash-tag creates a tetrameric fluorescent fusion protein X-FP and an oligomeric fusion protein Y-Ash respectively. Because each fusion protein has multiple Xs or Ys, the interaction between X and Y causes phase-separated droplets where the fluorescence by X-FP is concentrated and detectable as fluorescent puncta.