Product Description
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones.
For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 229
| Product Specifications |
| Application |
FC |
| Reactivity |
Mouse |
| Conjugation |
PE |
| Immunogen |
CEA |
| Peptide Sequence |
EAQNTTYL |
| Class |
Class I |
| Sequence Location |
526-533 |
| MHC Allele |
H-2Db |
| Citations for T-Select H-2Db CEA Tetramer-EAQNTTYL-PE – 1 Found |
| Kitadani, Junya; Ojima, Toshiyasu; Iwamoto, Hiromitsu; Tabata, Hirotaka; Nakamori, Mikihito; Nakamura, Masaki; Hayata, Keiji; Katsuda, Masahiro; Miyajima, Masayasu; Yamaue, Hiroki. Cancer Vaccine Therapy Using Carcinoembryonic Antigen - expressing Dendritic Cells generated from Induced Pluripotent Stem Cells. Scientific Reports. 2018;8(1):4569. PubMed |
