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Kohjin Bio




This Product is a medium for producing exosome derived from mesenchymal stem cell. After mesenchymal stem cells are grown in cell culture medium, they can be replaced
with KBM EV Pure to efficiently produce exosomes. Any type of cell growth medium is acceptable.

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| Immediately after medium replacement | After 48 hours of maintenance |
Fig.1 Morphology of cells maintained in KBM EV Pure
Purified 9mL of supernatant fluid sample
Lane(A) PC(Human-derived)
Lane(B) Culture supernatant (before purification)
Lane2 to 9 Elution fraction#2 to 9 1.0mL
Lane1 and 10 Elution fraction#1 and #10 0.5mL

Sample 2-10 mixed and concentrated (100 μL collection)
Lane(C) Before Concentration (Elution fractions 2-10 mix)
Lane(D) After Concentration (Elution fractions 2-10 mix)
Fig. 2:Surface marker expression
Fig. 3:Particle concentration and size distribution of EVs
Data Provided by:Prof. Kazuya Edamura,(DVM, PhD), Department of Veterinary Medicine, Nihon University
| Documents & Links for KBM EV Pure | |
| Datasheet | KBM EV Pure Datasheet |
| Documents & Links for KBM EV Pure | |
| Datasheet | KBM EV Pure Datasheet |
Kohjin Bio




This Product is a medium for producing exosome derived from mesenchymal stem cell. After mesenchymal stem cells are grown in cell culture medium, they can be replaced
with KBM EV Pure to efficiently produce exosomes. Any type of cell growth medium is acceptable.

![]() |
![]() |
| Immediately after medium replacement | After 48 hours of maintenance |
Fig.1 Morphology of cells maintained in KBM EV Pure
Purified 9mL of supernatant fluid sample
Lane(A) PC(Human-derived)
Lane(B) Culture supernatant (before purification)
Lane2 to 9 Elution fraction#2 to 9 1.0mL
Lane1 and 10 Elution fraction#1 and #10 0.5mL

Sample 2-10 mixed and concentrated (100 μL collection)
Lane(C) Before Concentration (Elution fractions 2-10 mix)
Lane(D) After Concentration (Elution fractions 2-10 mix)
Fig. 2:Surface marker expression
Fig. 3:Particle concentration and size distribution of EVs
Data Provided by:Prof. Kazuya Edamura,(DVM, PhD), Department of Veterinary Medicine, Nihon University
| Documents & Links for KBM EV Pure | |
| Datasheet | KBM EV Pure Datasheet |
| Documents & Links for KBM EV Pure | |
| Datasheet | KBM EV Pure Datasheet |