OptiPrep - Density Gradient Media (Iodixanol)Serumwerk Bernburg AGPrice from $194.00 to $1,739.00.
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Serumwerk Bernburg AG
Polysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. Concentrations up to 50% (w/v) can easily be obtained. The product is similar to Ficoll® 400.
The reactivity and stability of Polysucrose™ 400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. Polysucrose™ is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, Polysucrose™ 400 can be sterilized by autoclaving at 110°C for 30 minutes without any degradation.
Using sodium metrizoate and a polysaccharide Bøyum (1968) developed a one-step centrifugal technique for isolation of lymphocytes (Lymphoprep™). In this method the polysaccharide aggregates the erythrocytes, thereby increasing their sedimentation rate. Polysucrose ™ 400 has also been used as a density gradient medium for the purification of other cells and in membrane fractionation. Non-ionic high molecular weight solutes such as polysucrose are required for a number of other research scenarios
Polysucrose™ 400 is the ideal medium for:
| Documents & Links for Polysucrose 400 | |
| MSDS | Polysucrose 400 MSDS |
| Datasheet | Polysucrose 400 Datasheet |
| Documents & Links for Polysucrose 400 | |
| MSDS | Polysucrose 400 MSDS |
| Datasheet | Polysucrose 400 Datasheet |
Serumwerk Bernburg AG
Polysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. Concentrations up to 50% (w/v) can easily be obtained. The product is similar to Ficoll® 400.
The reactivity and stability of Polysucrose™ 400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. Polysucrose™ is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, Polysucrose™ 400 can be sterilized by autoclaving at 110°C for 30 minutes without any degradation.
Using sodium metrizoate and a polysaccharide Bøyum (1968) developed a one-step centrifugal technique for isolation of lymphocytes (Lymphoprep™). In this method the polysaccharide aggregates the erythrocytes, thereby increasing their sedimentation rate. Polysucrose ™ 400 has also been used as a density gradient medium for the purification of other cells and in membrane fractionation. Non-ionic high molecular weight solutes such as polysucrose are required for a number of other research scenarios
Polysucrose™ 400 is the ideal medium for:
| Documents & Links for Polysucrose 400 | |
| MSDS | Polysucrose 400 MSDS |
| Datasheet | Polysucrose 400 Datasheet |
| Documents & Links for Polysucrose 400 | |
| MSDS | Polysucrose 400 MSDS |
| Datasheet | Polysucrose 400 Datasheet |